Peptide Reconstitution Calculator
Calculate the exact volume of bacteriostatic water needed to achieve your target concentration, with per-dose measurements for insulin syringe administration.
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How to reconstitute peptides
Peptide reconstitution is the process of dissolving a lyophilised (freeze-dried) peptide powder in bacteriostatic water (BAC Water) to create a solution suitable for research administration. The concentration you achieve determines the volume required per dose.
The reconstitution formula
Concentration (mg/ml) = Peptide amount (mg) ÷ BAC Water volume (ml). A 5mg vial reconstituted with 2ml BAC Water yields a 2.5mg/ml solution. To administer 250mcg, you would draw 0.1ml.
Choosing your BAC Water volume
Most researchers use 1–2ml for standard 5mg vials. Smaller volumes give higher concentrations and smaller injection volumes. Larger volumes are easier to measure accurately with an insulin syringe but require more precise pipetting.
Insulin syringe reference
U-100 insulin syringes are calibrated to 100 units per ml. If your concentration is 2.5mg/ml and your dose is 250mcg (0.25mg), you need 0.1ml — which reads as 10 units on a U-100 syringe.
Storage after reconstitution
Reconstituted peptides should be stored at 2–8°C (standard refrigerator) and used within 28–30 days. Lyophilised peptides prior to reconstitution can be stored at -20°C for extended periods.
Peptide-specific reconstitution notes
BPC-157 (5mg): Typically reconstituted in 1–2ml BAC Water. Standard research concentrations range from 250mcg/ml to 1mg/ml.
TB-500 (5mg): Reconstitute in 1–2ml BAC Water. TB-500 (TB4 fragment) dissolves readily. Common research concentrations: 500mcg/ml to 2.5mg/ml.
Retatrutide (10mg / 20mg): GLP-1/GIP/glucagon triple agonist. Typically reconstituted in 2ml BAC Water for the 10mg vial (5mg/ml) or 4ml for the 20mg vial.
Ipamorelin (5mg): GHRP, commonly reconstituted in 2ml BAC Water giving 2.5mg/ml. Research doses typically in the 100–300mcg range.
GHK-Cu (50mg): Copper peptide, higher dose vial. Reconstitute in 5–10ml BAC Water depending on target concentration.